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<article dtd-version="1.1d2"> <front> <journal-meta>...</journal-meta> <article-meta> ... <volume>97</volume> <issue>4</issue> <fpage>1665</fpage> <lpage>1670</lpage> <history>...</history> <permissions> <copyright-statement>Copyright © 2000, The National Academy of Sciences</copyright-statement> </permissions> <abstract> <p>We describe a method for cloning nucleic acid molecules onto the surfaces of 5-μm microbeads rather than in biological hosts. A unique tag sequence is attached to each molecule, and the tagged library is amplified. Unique tagging of the molecules is achieved by sampling a small fraction (1%) of a very large repertoire of tag sequences. The resulting library is hybridized to microbeads that each carry ≈10<sup>6</sup> strands complementary to one of the tags. About 10<sup>5</sup> copies of each molecule are collected on each microbead. Because such clones are segregated on microbeads, they can be operated on simultaneously and then assayed separately. To demonstrate the utility of this approach, we show how to label and extract microbeads bearing clones differentially expressed between two libraries by using a fluorescence-activated cell sorter (FACS). Because no prior information about the cloned molecules is required, this process is obviously useful where sequence databases are incomplete or nonexistent. More importantly, the process also permits the isolation of clones that are expressed only in given tissues or that are differentially expressed between normal and diseased states. Such clones then may be spotted on much more cost-effective, tissue- or disease-directed, low-density planar microarrays.</p></abstract> <kwd-group kwd-group-type="author">...</kwd-group> </article-meta> </front> ... </article>
<article dtd-version="1.1d2"> <front> <article-meta> ... <permissions> <copyright-statement>Copyright © 1999, British Medical Journal</copyright-statement> <copyright-year>1999</copyright-year> </permissions> <abstract abstract-type="section"> <sec> <title>Objective</title> <p>To examine the effectiveness of day hospital attendance in prolonging independent living for elderly people.</p></sec> <sec> <title>Design</title> <p>Systematic review of 12 controlled clinical trials (available by January 1997) comparing day hospital care with comprehensive care (five trials), domiciliary care (four trials), or no comprehensive care (three trials).</p> </sec> <sec> <title>Subjects</title> <p>2867 elderly people.</p> </sec> <sec> <title>Main outcome measures</title> <p>Death, institutionalisation, disability, global “poor outcome,” and use of resources.</p> </sec> <sec> <title>Results</title> <p>Overall, there was no significant difference between day hospitals and alternative services ...</p> </sec> <sec> <title>Conclusions</title> <p>Day hospital care seems to be an effective service for elderly people ...</p> <p><boxed-text position="float"> <sec> <title>Key messages</title> <p>...</p> </sec> </boxed-text></p> </sec> </abstract> </article-meta> </front> ... </article>
... <article-meta> ... <abstract> <p>This is the third and last part of the volume devoted to solubility data of rare earth metal chlorides in water and in ternary and quaternary aqueous systems. Compilations of all available experimental data for each rare earth metal chloride are introduced with a corresponding critical evaluation. Every such evaluation contains a tabulated collection of all solubility results in water, a scheme of the water-rich part of the equilibrium ... Because the ternary and quaternary systems were almost never studied more than once, no critical evaluations or systematic comparisons of such data were possible. Simple chlorides (no complexes) of Gd, Tb, Dy, Ho, Er, Tm, Yb, and Lu are treated as the input substances. The literature (including a thorough coverage of papers in Chinese and Russian) has been covered through the middle of 2008.</p> </abstract> <abstract abstract-type="short"> <p>The is the third and last part of the volume devoted to solubility data of rare earth metal chlorides in water and in ternary and quaternary aqueous systems. Compilations of all available experimental data are introduced for each rare earth metal chloride with a corresponding critical evaluation. This part covers chlorides of Gd, Tb, Dy, Ho, Er, Tm, Yb, and Lu, with coverage of the literature through the middle of 2008.</p> </abstract> </article-meta> ...
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